Seller Name: RAYMOND T
Differential-Display Reverse Transcription-Pcr (Ddrt-Pcr) (Paperback)

Healthcare, Lab & Life Science

Colchester, CT 06415



ITEM#: 14728697

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.
Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.


ISBN 9783540632979
Genre SCIENCE / Life Sciences / Cell Biology
Format Paperback
Pages 124
Publisher Date 1998-01-01 00:00:00.0
Publisher Springer Verlag
Copyright Year 19980601
Height 9.5 in
Wdth 6.75 in
Thickness 0.5 in
Unit weight 0.6 lb
Language English
Series Name Springer Lab Manual
Audience College/higher education
Authors Colonna-Romano, Sergio
Country of Origin United States
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